High throughput estimates of Wolbachia, Zika and chikungunya infection in Aedes aegypti by near-infrared spectroscopy to improve arbovirus surveillance

• DOI: 10.1038/s42003-020-01601-0
• Publication Date: 2021-01-15T11:02:30Z
• Authors: Lilha M. B. Santos; Mathijs Mutsaers; Gabriela A. Garcia; Mariana R. David; Márcio G. Pavan; Martha T. Petersen; Jessica Corrêa-An...; Dinair Couto-Lima; Louis Maes; Floyd Dowell; Anton Lord; Maggy Sikulu-Lord; Rafael Maciel-de-...
• Keywords: Zika Virus

Abstract Deployment of Wolbachia to mitigate dengue (DENV), Zika (ZIKV) and chikungunya (CHIKV) transmission is ongoing in 12 countries. One way assess the efficacy releases determine invasion rates within wild population Aedes aegypti following their release. Herein we evaluated accuracy, sensitivity specificity Near Infrared Spectroscopy (NIRS) estimating time post death, ZIKV-, CHIKV-, -infection trapped dead female Ae. mosquitoes over a period 7 days. Regardless infection type, post-death was accurately predicted into four categories (fresh, 1 day old, 2–4 days old 5–7 old). Overall accuracies 93.2, 97 90.3% were observed when NIRS used detect ZIKV, CHIKV indicating could be potentially applied as rapid cost-effective arbovirus surveillance tool. However, field data required demonstrate full capacity for detecting these infections under conditions.