Megahertz single-particle imaging at the European XFEL

• DOI: 10.1038/s42005-020-0362-y
• Publication Date: 2020-05-29T10:02:27Z
• Authors: Egor Sobolev; Sergei Zolotarev; Klaus Giewekemeyer; Johan Bielecki; Kenta Okamoto; Hemanth K. N. Reddy; Jakob Andreasson; Kartik Ayyer; Imrich Barak; Sadia Bari; Anton Barty; Richard Bean; Sergey Bobkov; Henry N. Chapman; Grzegorz Chojnowski; Benedikt J. Daurer; Katerina Dörner; Tomas Ekeberg; Leonie Flückiger; Oxana Galzitskaya; Luca Gelisio; Steffen Hauf; Brenda G. Hogue; Daniel A. Horke; Ahmad Hosseinizadeh; Vyacheslav Ilyin; Chulho Jung; Chan Kim; Yoonhee Kim; Richard A. Kirian; Henry Kirkwood; Olena Kulyk; Jochen Küpper; Romain Letrun; N. Duane Loh; Kristina Lorenzen; Marc Messerschmidt; Kerstin Mühlig; Abbas Ourmazd; Natascha Raab; Andrei V. Rode; Max Rose; Adam Round; Takushi Sato; Robin Schubert; Peter Schwander; Jonas A. Sellberg; Marcin Sikorski; Alessandro Silenzi; Changyong Song; John C. H. Spence; Stephan Stern; Jolanta Sztuk-Dam...; Anthon Teslyuk; Nicusor Timneanu; Martin Trebbin; Charlotte Uetrecht; Britta Weinhausen; Garth J. Williams; P. Lourdu Xavier; Chen Xu; Ivan A. Vartanyants; Victor S. Lamzin; Adrian Mancuso; Filipe R. N. C. Maia
• Keywords: Repetition (rhetorical device); Particle (ecology); Pulse repetition frequency

Abstract The emergence of high repetition-rate X-ray free-electron lasers (XFELs) powered by superconducting accelerator technology enables the measurement significantly more experimental data per day than was previously possible. European XFEL is expected to provide 27,000 pulses second, over two orders magnitude any other XFEL. increased pulse rate a key enabling factor for single-particle diffractive imaging, which relies on averaging weak diffraction signal from single biological particles. Taking full advantage this new capability requires that all steps, sample preparation and delivery acquisition patterns, are compatible with repetition rate. Here, we show imaging can be performed using at megahertz rates. results obtained pave way towards exploiting their