Multilevel X-ray imaging approach to assess the sequential evolution of multi-organ damage in multiple sclerosis

• DOI: 10.1038/s42005-022-01070-3
• Publication Date: 2022-11-18T09:43:51Z
• Authors: Francesca Palermo; Nicola Pieroni; Alessia Sanna; Benedetta Parodi; Consuelo Venturi; Ginevra Begani Pr...; Lorenzo Massimi; Laura Maugeri; Gian Paolo Marra; Elena Longo; Lorenzo D’Amico; Giulia Saccomano; Jonathan Perrin; Giuliana Tromba; Inna Bukreeva; Michela Fratini; Giuseppe Gigli; Nicole Kerlero de...; Alessia Cedola
• Keywords: Organ system; Demyelinating disease

Abstract The 3D complexity of biological tissues and intricate structural-functional connections call for state-of-the-art X-ray imaging approaches to overcome limitations classical imaging. Unlike other techniques, phase-contrast tomography (XPCT) offers a highly sensitive approach investigate different disease-relevant networks at levels ranging from single cell through intact organ. We present here concomitant study the evolution tissue damage inflammation in organs affected by disease murine model multiple sclerosis, demyelinating autoimmune disorder central nervous system. XPCT identifies monitors structural cellular alterations throughout system, but also gut, eye, mice induced develop sclerosis-like sacrificed pre-symptomatic symptomatic time points. This details sequential multi-organ damages sclerosis showing development progression which is relevance human case.