A dynamic view of the proteomic landscape during differentiation of ReNcell VM cells, an immortalized human neural progenitor line

• DOI: 10.1038/sdata.2019.16
• Publication Date: 2019-02-19T14:03:27Z
• Authors: Yuyu Song; Kartik Subramanian; Matthew J. Berberich; Steven Rodriguez; Isabel J. Latorre; Catherine M. Luria; Robert Everley; Mark W. Albers; Timothy J. Mitchison; Peter K. Sorger
• Keywords: Progenitor; High-Content Screening; Immortalised cell line

Abstract The immortalized human ReNcell VM cell line represents a reproducible and easy-to-propagate culture system for studying the differentiation of neural progenitors. To better characterize starting its subsequent differentiation, we assessed protein phospho-protein levels morphology over 15-day period during which progenitors differentiated into neurons, astrocytes oligodendrocytes. Five resulting datasets measured or states phosphorylation based on tandem-mass-tag (TMT) mass spectrometry four characterized cellular phenotypes using high-content microscopy. Proteomic analysis revealed changes in pathways responsible cytoskeletal rearrangement, phase transitions, neuronal migration, glial neurotrophic signalling extracellular matrix regulation. imaging data accelerated cells treated with poly-selective CDK GSK3 inhibitor kenpaullone HMG-CoA reductase mevastatin, both have previously been reported to promote differentiation. These provide in-depth information progenitor state presence absence drugs, setting stage functional studies.