A metagenomic survey of soil microbial communities along a rehabilitation chronosequence after iron ore mining

• DOI: 10.1038/sdata.2019.8
• Publication Date: 2019-02-12T14:02:56Z
• Authors: Markus Gastauer; Mabel Patricia Or...; Kleber Padovani d...; Eder Soares Pires; Ronnie Alves; Cecílio Frois Cal...; Silvio Junio Ramos; Guilherme Oliveira
• Keywords: Chronosequence; Environmental DNA

Abstract Microorganisms are useful environmental indicators, able to deliver essential insights processes regarding mine land rehabilitation. To compare microbial communities from a chronosequence of rehabilitation pre-disturbance levels references sites covered by native vegetation, we sampled non-rehabilitated, rehabilitating and reference study the Urucum Massif, Southwestern Brazil. From each site, three composed soil samples were collected for chemical, physical, metagenomics analysis. We used paired-end library sequencing technology (NextSeq 500 Illumina); reads assembled using MEGAHIT. Coding DNA sequences (CDS) identified Kaiju in combination with non-redundant NCBI BLAST containing archaea, bacteria, viruses. Additionally, functional classification was performed EMG v2.3.2. Here, provide raw data assembly (reads contigs), followed initial taxonomic analysis, as base-line further studies this kind. Further investigation is needed fully understand mechanisms tropical regions, inspiring researchers explore collection hypothesis testing.