Pre-erythrocytic antibody profiles induced by controlled human malaria infections in healthy volunteers under chloroquine prophylaxis

• DOI: 10.1038/srep03549
• Publication Date: 2013-12-19T10:16:00Z
• Authors: Philip L. Felgner; Meta Roestenberg; Li Liang; Christopher Hung; Aarti Jain; Jozelyn Pablo; Rie Nakajima-Sasaki; Douglas Molina; Karina Teelen; Cornelus C. Hermsen; Robert Sauerwein
• Keywords: Malaria prophylaxis

Abstract Complete sterile protection to Plasmodium falciparum ( Pf ) infection mediated by pre-erythrocytic immunity can be experimentally induced under chloroquine prophylaxis, through immunization with sporozoites from infected mosquitoes' bites (CPS protocol). To characterize the profile of CPS antibody (Ab) responses, we developed a proteome microarray containing 809 antigens showing distinct Ab recognition expressed in life-cycle stages. In contrast, plasma naturally exposed semi-immune individuals Kenya was skewed toward reactivity against asexual blood stage antigens. CPS-immunized and generated antibodies 192 202 antigens, respectively, but only 60 overlapped between two groups. Although number reactive varied individuals, all volunteers reacted strongly circumsporozoite protein (CSP) liver antigen 1 (LSA1). Well classified merozoite erythrocytic were lacking immunized group. These data show that groups have quite profiles reflecting their protective immunity; react while mainly