Minimally destructive sampling of type specimens of Pyropia (Bangiales, Rhodophyta) recovers complete plastid and mitochondrial genomes

• DOI: 10.1038/srep05113
• Publication Date: 2014-06-04T09:02:40Z
• Authors: Jeffery R. Hughey; Paul W. Gabrielson; Laurence Rohmer; Jacquie Tortolani; Mayra Silva; Kathy Ann Miller; Joel D. Young; Craig Martell; Erik Ruediger
• Keywords: 0301 basic medicine; Base Sequence; Genome, Plastid; Molecular Sequence Data; Chromosome Mapping; Article; Specimen Handling; 03 medical and health sciences; Natural Variation; Genomic Analysis; Genome, Mitochondrial; Rhodophyta; Genome, Plant

Plant species, including algae and fungi, are based on type specimens to which the name of a taxon is permanently attached. Applying scientific any specimen therefore requires demonstrating correspondence between that specimen. Traditionally, identifications morpho-anatomical characters, but recently systematists using DNA sequence data. These studies flawed if isolated from misidentified modern specimens. We propose genome-based solution. Using 4 × mm2 material specimens, we assembled 14 plastid 15 mitochondrial genomes attributed red Pyropia perforata, Py. fucicola kanakaensis. The chloroplast were fairly conserved, differed significantly among populations in content length. Complete attainable 19th early 20th century specimens; this validates effort cost their curation as well supports practice method.