Engineering an in vitro air-blood barrier by 3D bioprinting

• DOI: 10.1038/srep07974
• Publication Date: 2015-01-22T10:16:50Z
• Authors: Lenke Horváth; Yuki Umehara; Corinne Jud; Fabian Blank; Alke Petri-Fink; Barbara Rothen-Ru...
• Keywords: 3D bioprinting; Biofabrication

Intensive efforts in recent years to develop and commercialize vitro alternatives the field of risk assessment have yielded new promising two- three dimensional (3D) cell culture models. Nevertheless, a realistic 3D alveolar model is not available yet. Here we report on biofabrication human air-blood tissue barrier analogue composed an endothelial cell, basement membrane epithelial layer by using bioprinting technology. In contrary manual method, demonstrate that this technique enables automatized reproducible creation thinner more homogeneous layers, which required for optimal barrier. This platform will offer excellent tool engineer advanced lung high-throughput screening safety drug efficacy testing.