Integrative analysis of differentially expressed microRNAs of pulmonary alveolar macrophages from piglets during H1N1 swine influenza A virus infection

• DOI: 10.1038/srep08167
• Publication Date: 2015-02-02T10:31:37Z
• Authors: Pengfei Jiang; Na Zhou; Xinyu Chen; Xing Zhao; Dengyun Li; Fen Wang; Lijun Bi; Deli Zhang
• Keywords: Pathogenesis; H1N1 influenza

Abstract H1N1 swine influenza A virus (H1N1 SwIV) is one key subtype of viruses with pandemic potential. MicroRNAs (miRNAs) are endogenous small RNA molecules that regulate gene expression. MiRNAs relevant SwIV have rarely been reported. To understand the biological functions miRNAs during infection, this study profiled differentially expressed (DE) in pulmonary alveolar macrophages from piglets infection using a deep sequencing approach, which was validated by quantitative real-time PCR. Compared to control group, 70 and 16 DE were respectively identified on post-infection day (PID) 4 PID 7. 56 between Our results suggest most host down-regulated defend acute phase whereas their expression levels gradually return normal recovery avoid occurrence too severe porcine lung damage. In addition, targets also obtained, for bioinformatics analyses performed. would be useful investigating regulatory mechanisms human because pig serves as an excellent animal model pathogenesis influenza.