MicroRNA-26a prevents endothelial cell apoptosis by directly targeting TRPC6 in the setting of atherosclerosis

• DOI: 10.1038/srep09401
• Publication Date: 2015-03-24T05:39:13Z
• Authors: Yong Zhang; Wei Qin; Longyin Zhang; Xianxian Wu; Ning Du; Yingying Hu; Xiaoguang Li; Nannan Shen; Dan Xiao; Haiying Zhang; Zhange Li; Yue Zhang; Huan Yang; Feng Gao; Zhimin Du; Chaoqian Xu; Baofeng Yang
• Keywords: TRPC6

Abstract Atherosclerosis, a chronic inflammatory disease, is the major cause of life-threatening complications such as myocardial infarction and stroke. Endothelial apoptosis plays vital role in initiation progression atherosclerotic lesions. Although subset microRNAs (miRs) have been identified critical regulators atherosclerosis, studies on their participation endothelial atherosclerosis limited. In our study, we found that miR-26a expression was substantially reduced aortic intima ApoE −/− mice fed with high-fat diet (HFD). Treatment human cells (HAECs) oxidized low-density lipoprotein (ox-LDL) suppressed expression. Forced inhibited evidenced by MTT assay TUNEL staining results. Further analysis TRPC6 target overexpression abolished anti-apoptotic effect miR-26a. Moreover, cytosolic calcium mitochondrial apoptotic pathway were to mediate beneficial effects apoptosis. Taken together, study reveals novel indicates therapeutic potential for associated cell death.