Efficient in vitro generation of functional thymic epithelial progenitors from human embryonic stem cells

• DOI: 10.1038/srep09882
• Publication Date: 2015-06-05T11:30:50Z
• Authors: Min Su; Rong Hu; Jingjun Jin; Yuan Yan; Yinhong Song; Ryan Sullivan; Laijun Lai
• Keywords: 0303 health sciences; Lymphopoiesis; T-Lymphocytes; Human Embryonic Stem Cells; Cell Culture Techniques; Mice, Nude; Cell Differentiation; Epithelial Cells; Thymus Gland; In Vitro Techniques; Article; Mice; 03 medical and health sciences; Animals; Humans; Stem Cell Transplantation

Abstract Thymic epithelial cells (TECs) are the major components of thymic microenvironment for T cell development. TECs derived from progenitors (TEPs). It has been reported that human ESCs (hESCs) can be directed to differentiate into TEPs in vitro . However, efficiency differentiation is low. Furthermore, transplantation hESC-TEPs mice only resulted a very low level development co-transplanted hematopoietic precursors. We show here we have developed novel protocol efficiently induce hESCs When transplanted mice, develop and form architecture. Most importantly, hESC-TECs support long-term functional mouse or higher The may provide new approach prevent treat patients with immunodeficiency.