Active macropinocytosis induction by stimulation of epidermal growth factor receptor and oncogenic Ras expression potentiates cellular uptake efficacy of exosomes

• DOI: 10.1038/srep10300
• Publication Date: 2015-06-03T11:54:31Z
• Authors: Ikuhiko Nakase; Nahoko Bailey Kob...; Tomoka Takatani-N...; Tetsuhiko Yoshida
• Keywords: Pinocytosis; Membrane ruffling

Abstract Exosomes are approximately 100-nm vesicles that consist of a lipid bilayer cellular membranes secreted in large quantities from various types normal and disease-related cells. Endocytosis has been reported as major pathway for the uptake exosomes; however, detailed mechanisms their still unknown. Here, we demonstrate active induction macropinocytosis (accompanied by actin reorganisation, ruffling plasma membrane engulfment volumes extracellular fluid) stimulation cancer-related receptors show epidermal growth factor (EGF) receptor significantly enhances exosomes. We also oncogenic K-Ras-expressing MIA PaCa-2 cells exhibit intensive actively transports exosomes into compared with wild-type BxPC-3 Furthermore, encapsulation ribosome-inactivating protein saporin EGF using our simple electroporation method produces superior cytotoxicity via enhanced Our findings contribute to biological, pharmaceutical medical research fields terms understanding macropinocytosis-mediated applications exosomal delivery systems.