Regulation of NDR1 activity by PLK1 ensures proper spindle orientation in mitosis

• DOI: 10.1038/srep10449
• Publication Date: 2015-06-09T13:48:54Z
• Authors: Maomao Yan; Lingluo Chu; Bo Qin; Zhikai Wang; Xing Liu; Changjiang Jin; Guanglan Zhang; Marta Gomez; Alexander Hergovich; Zhengjun Chen; Ping He; Xinjiao Gao; Xuebiao Yao
• Keywords: PLK1; Spindle pole body; Mitotic exit; Polo-like kinase; Spindle checkpoint; Multipolar spindles

Accurate chromosome segregation during mitosis requires the physical separation of sister chromatids which depends on correct position mitotic spindle relative to membrane cortex. Although recent work has identified role PLK1 in orientation, mechanisms underlying signaling positioning and orientation have not been fully illustrated. Here, we a conserved axis NDR1 kinase activity is regulated by mitosis. phosphorylates at three putative threonine residues (T7, T183 T407) entry, elicits PLK1-dependent suppression ensures Importantly, persistent expression non-phosphorylatable mutant perturbs orientation. Mechanistically, PLK1-mediated phosphorylation protects binding Mob1 subsequent activation. These findings define that integrates dynamic kinetochore-microtubule interaction control genomic stability maintenance.