Effects of Exendin-4 on bone marrow mesenchymal stem cell proliferation, migration and apoptosis in vitro

• DOI: 10.1038/srep12898
• Publication Date: 2015-08-07T09:02:56Z
• Authors: Hao Zhou; Dandan Li; Chen Shi; Ting Xin; Junjie Yang; Ying Zhou; Shunyin Hu; Feng Tian; Jing Wang; Yundai Chen
• Keywords: 0301 basic medicine; Receptors, CXCR4; Cell Survival; Venoms; Apoptosis; Bone Marrow Cells; Mesenchymal Stem Cells; Hydrogen Peroxide; Article; Mitochondria; Rats; Rats, Sprague-Dawley; Phosphatidylinositol 3-Kinases; 03 medical and health sciences; Cell Movement; Animals; Exenatide; Apoptosis Regulatory Proteins; Peptides; Reactive Oxygen Species; Proto-Oncogene Proteins c-akt; Cell Proliferation; Signal Transduction

Abstract Mesenchymal stem cells (MSC) are regarded as an attractive source of therapeutic for myocardial infarction. However, their limited self-renewal capacity, low migration capacity and poor viability after transplantation hamper the clinical use MSC; thus, a strategy to enhance biological functions MSC is required. Exendin-4 (Ex-4), glucagon-like peptide-1 receptor agonist, exerts cell-protective effects on many types cells. little information available regarding influence Ex-4 MSC. In our study, were isolated from bone marrow cultured in vitro. After treatment with Ex-4, displayed higher proliferative increased C-X-C motif 4 (CXCR4) expression enhanced response. Moreover, H 2 O -induced apoptosis, preserved mitochondrial function through scavenging ROS balancing anti- pro-apoptotic proteins, leading inhibition mitochondria-dependent cell death pathways survival. phospho-Akt (p-Akt) was observed intervention. blockade PI3K/Akt pathway inhibitors suppressed above cytoprotective suggesting that partly responsible Ex-4-mediated growth, mobilization These findings provide method maximizing effectiveness MSC-based therapies applications.