Investigation on the effects of the atmospheric pressure plasma on wound healing in diabetic rats

• DOI: 10.1038/srep19144
• Publication Date: 2016-02-23T12:07:32Z
• Authors: Sara Fathollah; Shahriar Mirpour; Parvin Mansouri; Ahmad Reza Dehpour; Mahmood Ghoranneviss; Nastaran Rahimi; Zahra Safaie Naraghi; Reza Chalangari; Katalin Martits C...
• Keywords: Male; Wound Healing; 0303 health sciences; Plasma Gases; Gene Expression; Neovascularization, Physiologic; Wounds, Nonpenetrating; Helium; Article; Streptozocin; Diabetes Mellitus, Experimental; Rats; 3. Good health; Transforming Growth Factor beta1; 03 medical and health sciences; Animals; Collagen; Biomarkers; Skin

Abstract It is estimated that 15 percent of individuals with diabetes mellitus suffer from diabetic ulcers worldwide. The aim this study to present a non-thermal atmospheric plasma treatment as novel therapy for wounds. consists ionized helium gas produced by high-voltage (8 kV) and high-frequency (6 kHz) power supply. Diabetes was induced in rats via an intravascular injection streptozotocin. then introduced artificial xerograph wounds the 10 minutes. Immunohistochemistry assays performed determine level transforming growth factor (TGF-β1) cytokine. results showed low healing rate compared wound-healing non-diabetic animals (P < 0.05). Moreover, noted enhanced 0.05) significant wound contraction occurred after untreated Histological analyses revealed formation epidermis layer, neovascularization cell proliferation. also resulted release TGF-β1 cytokine cells tissue medium. findings demonstrate effect rats.