Epigallocatechin-3-O-gallate up-regulates microRNA-let-7b expression by activating 67-kDa laminin receptor signaling in melanoma cells

• DOI: 10.1038/srep19225
• Publication Date: 2016-01-12T10:56:34Z
• Authors: Shuhei Yamada; Shuntaro Tsukamoto; Yuhui Huang; Akiko Makio; Motofumi Kumazoe; Shuya Yamashita; Hirofumi Tachibana
• Keywords: HMGA2

MicroRNAs (miRNAs) are non-coding RNAs involved in various biological processes by regulating their target genes. Green tea polyphenol (-)-epigallocatechin-3-O-gallate (EGCG) inhibits melanoma tumor growth activating 67-kDa laminin receptor (67LR) signaling. To examine the effect of EGCG on miRNA expression cells, we performed microarray analysis. We showed that up-regulated miRNA-let-7b through 67LR cells. The EGCG-induced up-regulation let-7b led to down-regulation high mobility group A2 (HMGA2), a gene related progression. 67LR-dependent cAMP/protein kinase A (PKA)/protein phosphatase 2A (PP2A) signaling pathway activation was induced EGCG. These findings provide basis for understanding mechanism regulation