Characterization of the promoter region of the bovine long-chain acyl-CoA synthetase 1 gene: Roles of E2F1, Sp1, KLF15 and E2F4

• DOI: 10.1038/srep19661
• Publication Date: 2016-01-19T09:32:19Z
• Authors: Zhi-Dong Zhao; Lin-Sen Zan; An-Ning Li; Gong Cheng; Shi-Jun Li; Ya-Ran Zhang; Xiao-Yu Wang; Ying-Ying Zhang
• Keywords: 2. Zero hunger; 0303 health sciences; Base Sequence; Transcription, Genetic; Sp1 Transcription Factor; DNA Mutational Analysis; Kruppel-Like Transcription Factors; E2F4 Transcription Factor; Lipids; Article; Red Meat; 03 medical and health sciences; Adipose Tissue; Coenzyme A Ligases; Fatty Acids, Unsaturated; Animals; Cattle; CpG Islands; Transcription Initiation Site; Muscle, Skeletal; Promoter Regions, Genetic; E2F1 Transcription Factor; Protein Binding; Sequence Deletion

Abstract The nutritional value and eating qualities of beef are enhanced when the unsaturated fatty acid content fat is increased. Long-chain acyl-CoA synthetase 1 (ACSL1) plays key roles in transport degradation, as well lipid synthesis. It has been identified a plausible functional positional candidate gene for manipulations composition bovine skeletal muscle. In present study, we determined that ACSL1was highly expressed subcutaneous adipose tissue longissimus thoracis . To elucidate molecular mechanisms involved ACSL1 regulation, cloned characterized promoter region ACSL1. Applying 5′-rapid amplification cDNA end analysis (RACE), multiple transcriptional start sites (TSSs) its region. Using series 5′ deletion plasmids luciferase reporter assays, found proximal minimal was located within −325/−141 relative to TSS it also predicted CpG island. Mutational electrophoretic mobility shift assays demonstrated E2F1, Sp1, KLF15 E2F4 binding drives transcription. Together these interactions integrate frame role mediating beef.