Novel functional microRNAs from virus-free and infected Vitis vinifera plants under water stress

• DOI: 10.1038/srep20167
• Publication Date: 2016-02-02T11:36:21Z
• Authors: Vitantonio Pantaleo; Marco Vitali; Paolo Boccacci; Laura Miozzi; Danila Cuozzo; Walter Chitarra; Franco Mannini; Claudio Lovisolo; Giorgio Gambino
• Keywords: Biotic stress

Abstract MicroRNAs (miRNAs) are small non-coding RNAs that regulate the post-transcriptional control of several pathway intermediates, thus playing pivotal roles in plant growth, development and response to biotic abiotic stresses. In recent years, grapevine genome release, small(s)-RNAseq degradome-RNAseq together has allowed discovery characterisation many miRNA species, rendering additional miRNAs difficult uncertain. Taking advantage responsiveness stresses availability virus-free Vitis vinifera plants those infected only by a latent virus, we have analysed grapevines subjected drought greenhouse conditions. The sRNA-seq other sequence-specific molecular analyses us characterise conserved expression profiles association with specific eco-physiological parameters. addition, here report 12 novel grapevine-specific candidates describe their profile. We show viral infection can influence V. drought. Moreover, study parameters showed photosynthetic rate, stomatal conductance hydraulic resistance water transport were significantly influenced infection. Although no unequivocal cause–effect explanation could be attributed each target, contribution is discussed.