SmMYC2a and SmMYC2b played similar but irreplaceable roles in regulating the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza

• DOI: 10.1038/srep22852
• Publication Date: 2016-03-07T10:19:57Z
• Authors: Yangyun Zhou; Wei Sun; Junfeng Chen; Hexin Tan; Ying Xiao; Qing Li; Qian Ji; Shouhong Gao; Li Chen; Shilin Chen; Lei Zhang; Wansheng Chen
• Keywords: Salvia miltiorrhiza

Salvia miltiorrhiza Bunge, which contains tanshinones and phenolic acids as major classes of bioactive components, is one the most widely used herbs in traditional Chinese medicine. Production enhanced by methyl jasmonate (MeJA). Transcription factor MYC2 switch jasmontes signaling plants. Here, we focused on two novel JA-inducible genes S. miltiorrhiza, designated SmMYC2a SmMYC2b, were localized nucleus. SmMYC2b also discovered to interact with SmJAZ1 SmJAZ2, implying that MYC2s might function direct targets JAZ proteins. Ectopic RNA interference (RNAi)-mediated knockdown experiments suggested SmMYC2a/b affected multiple tanshinone acid biosynthetic pathway. Besides, accumulation was impaired loss SmMYC2a/b. Meanwhile, could bind an E-box motif within SmHCT6 SmCYP98A14 promoters, while bound promoter, through regulation pathway achieve. Together, these results suggest are JAZ-interacting transcription factors positively regulate biosynthesis Sal B similar but irreplaceable effects.