A critical role of mevalonate for peptidoglycan synthesis in Staphylococcus aureus

• DOI: 10.1038/srep22894
• Publication Date: 2016-03-10T10:59:45Z
• Authors: Yasuhiko Matsumoto; Jyunichiro Yasukawa; Masaki Ishii; Yohei Hayashi; Shinya Miyazaki; Kazuhisa Sekimizu
• Keywords: Mevalonate pathway

3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase, a mevalonate synthetase, is required for the growth of Staphylococcus aureus. However, essential role enzyme in cell has remained unclear. Here we show that three mutants possessed single-base substitutions mvaA gene, which encodes HMG-CoA temperature-sensitive phenotype. The phenotype was suppressed by addition or farnesyl diphosphate, product synthesized from mevalonate. Farnesyl diphosphate precursor undecaprenyl phosphate peptidoglycan synthesis. rate synthesis decreased under non-permissive conditions and reductase activities mutant MvaA proteins temperature sensitive were lower than wild-type protein. Our findings genetic biochemical analyses suggest produced S. aureus growth.