Dissecting Stages of Human Kidney Development and Tumorigenesis with Surface Markers Affords Simple Prospective Purification of Nephron Stem Cells

• DOI: 10.1038/srep23562
• Publication Date: 2016-03-29T14:01:52Z
• Authors: Naomi Pode-Shakked; Oren Pleniceanu; Rotem Gershon; Rachel Shukrun; Itamar Kanter; Efrat Bucris; Ben Pode-Shakked; Gal Tam; Hadar Tam; Revital Caspi; Sara Pri-Chen; Einav Vax; Guy Katz; Dorit Omer; Orit Harari-Stein...; Tomer Kalisky; Benjamin Dekel
• Keywords: Multipotent Stem Cell

Abstract When assembling a nephron during development multipotent stem cell pool becomes restricted as differentiation ensues. A faulty arrest in this process leads to transformation and initiation of Wilms’ tumor. Mapping these transitions with respective surface markers affords accessibility specific subpopulations. NCAM1 CD133 have been previously suggested mark human renal progenitor populations. Herein, using sorting, RNA sequencing, vitro studies serum-free media vivo xenotransplantation we demonstrate sequential map that links kidney tumorigenesis; In nephrogenesis, + − marks SIX2 cells transiting differentiating segment-specific epithelial progenitors differentiated cells. tumorigenesis, blastema includes the ALDH1 WT cancer stem/initiating cells, while specifying early late differentiation, are severely tumor capacity self-renewal. Thus, negative selection for is required defining normal malignant nephrogenesis.