Enoyl-CoA hydratase mediates polyhydroxyalkanoate mobilization in Haloferax mediterranei

• DOI: 10.1038/srep24015
• Publication Date: 2016-04-07T09:37:11Z
• Authors: Guiming Liu; Shuangfeng Cai; Jing Hou; Dahe Zhao; Jing Han; Jian Zhou; Hua Xiang
• Keywords: Haloferax volcanii; Haloarchaea

Abstract Although polyhydroxyalkanoate (PHA) accumulation and mobilization are one of the most general mechanisms for haloarchaea to adapt hypersaline environments with changeable carbon sources, PHA pathways still not clear any haloarchaea. In this study, functions five putative ( R )-specific enoyl-CoA hydratases (R-ECHs) in Haloferax mediterranei , named PhaJ1 PhaJ5, respectively, were thoroughly investigated. Through gene deletion complementation, we demonstrated that only certain these ECHs had a slight contribution poly(3-hydroxybutyrate- co -3-hydroxyvalerate) (PHBV) biosynthesis. But significantly, PhaJ1, R-ECH is associated granules, was shown be involved haloarchaeon. catalyzes dehydration )-3-hydroxyacyl-CoA, common product degradation, enoyl-CoA, intermediate β-oxidation cycle, thus could link pathway H. . This linkage further indicated from up-regulation key genes under condition, as well obvious inhibition degradation upon pathway. Interestingly, 96% phaJ -containing haloarchaeal species possess both phaC (encoding synthase) full set β-oxidation, implying storage through cycle would