Establishment and characterization of HROC69 – a Crohn´s related colonic carcinoma cell line and its matched patient-derived xenograft

• DOI: 10.1038/srep24671
• Publication Date: 2016-04-18T09:24:10Z
• Authors: Florian Kuehn; Christina S. Mullins; Mathias Krohn; Christine Harnack; Robert Ramer; Oliver H. Krämer; Ernst Klar; Maja Huehns; Michael Linnebacher
• Keywords: Chromosome Aberrations; Male; 0301 basic medicine; Adenomatous Polyposis Coli Protein; Carcinoma; Cell Culture Techniques; PTEN Phosphohydrolase; Genes, MHC Class I; Middle Aged; HCT116 Cells; Article; 3. Good health; Mice; 03 medical and health sciences; Crohn Disease; Colonic Neoplasms; Biomarkers, Tumor; Animals; Heterografts; Humans; Smad3 Protein; Tumor Suppressor Protein p53; Cells, Cultured

Abstract Colitis-associated colorectal cancer (CAC) seems to be a rather unique entity and differs in its genetic alterations, tumour formation capacities clinical features from sporadic carcinoma. Most descriptions about biology of CAC refer ulcerative colitis; data Crohn´s colitis related carcinomas are scarce. The majority patients with disease under immunosuppression which generates different environment for growth. We first describe the case fast growing long-term immunosuppressed patient successful establishment characterization carcinoma cell lines along their corresponding patient-derived xenograft. Subsequently, these tumor models were molecularly functionally analysed. Beside numerous chromosomal mutations TP53, APC, PTEN SMAD3 identified. express testis antigens, surface molecules involved immune evasion but low levels HLA class I molecules. They show strong invasive comparison weak migratory activity. present work is description vitro vivo patient. might valuable tools analysis epigenetic biomarker identification, functional testing, including response prediction development specific therapeutical strategies.