Impaired immunological synapse in sperm associated antigen 6 (SPAG6) deficient mice

• DOI: 10.1038/srep25840
• Publication Date: 2016-05-12T09:16:50Z
• Authors: Lauren Folgosa Co...; Mohey Eldin El Shikh; Wei Li; Rebecca C. Keim; Zhengang Zhang; Jerome F. Strauss; Zhibing Zhang; Daniel H. Conrad
• Keywords: Immunological synapse

Abstract Sperm associated antigen 6 (SPAG6), a component of the central apparatus “9 + 2” axoneme, plays role in ciliary and flagellar motility; but, its contribution to adaptive immunity immune system development is completely unknown. While cells lack cilium, immunological synapse surrogate cilium as it utilizes same machinery ciliogenesis including nucleation microtubules at centrosome. This prompted our hypothesis that SPAG6 critically regulates formation function synapses. Using bone marrow reconstitution studies adult WT mice, we demonstrate expressed primary secondary lymphoid tissues, with centrosome lymphocytes, deficiency results disruption due loss polarization actin clearance synaptic cleft. Improper Spag6 KO mice was defective CTL functions impaired humoral indicated by reduced germinal centers reactions, follicular CD4 T cells, production class-switched antibody, together expansion B1 B cells. novel report demonstrates requirement for optimal function, direct cell provides mechanism infertility disorders related SPAG6.