Analysis of miRNAs and their target genes associated with lipid metabolism in duck liver

• DOI: 10.1038/srep27418
• Publication Date: 2016-06-08T09:46:19Z
• Authors: Jun He; Weiqun Wang; Lizhi Lu; Yong Tian; Dong Niu; Jindong Ren; Liyan Dong; Siwei Sun; Yan Zhao; Li Chen; Jianliang Shen; Xiuhong Li
• Keywords: 0303 health sciences; Gene Expression Profiling; 500; Lipid Metabolism; Article; MicroRNAs; 03 medical and health sciences; Ducks; Liver; Animals; Gene Regulation; MiRNAs; RNA, Messenger

Abstract Fat character is an important index in duck culture that linked to local flavor, feed cost and fat intake for costumers. Since the regulation networks lipid metabolism had not been reported very clearly, we aimed explore potential miRNA-mRNA pairs their regulatory roles metabolism. Here, Cherry-Valley ducks were selected treated with/without 5% oil added 2 weeks, then content determination was performed on. The data showed contents fatty acid ratios of C17:1 C18:2 up-regulated livers oil-added ducks, while C12:0 ratio down-regulated. Then 21 differential miRNAs, including 10 novel obtain from by sequencing, 73 target genes involved metabolic processes these miRNAs found, which constituted 316 pairs. Two one miRNA known miRNA, N-miR-16020-FASN gga-miR-144-ELOVL6, validate negative relation. And results N-mir-16020 gga-miR-144 could respectively bind 3′-UTRs FASN ELOVL6 control expressions. This study provides new sights useful information future research on network