Rapid identification of fruit length loci in cucumber (Cucumis sativus L.) using next-generation sequencing (NGS)-based QTL analysis

• DOI: 10.1038/srep27496
• Publication Date: 2016-06-07T09:28:04Z
• Authors: Qing-zhen Wei; Wen-yuan Fu; Yun-zhu Wang; Xiao-dong Qin; Jing Wang; Ji Li; Qun-feng Lou; Jin-feng Chen
• Keywords: Cucumis; Family-based QTL mapping; Bulked segregant analysis; Candidate gene

The cucumber (Cucumis sativus L.) exhibits extensive variations in fruit size and shape. Fruit length is an important agronomic domesticated trait controlled by quantitative loci (QTLs). Nonetheless, the underlying molecular genetic mechanisms that determine remain unclear. QTL-seq efficient strategy for QTL identification takes advantage of bulked-segregant analysis (BSA) next-generation sequencing (NGS). In present study, we conducted mapping length. identified 8 QTLs immature mature A major-effect fl3.2, which explained a maximum 38.87% phenotypic variation, was detected. genome-wide comparison SNP profiles between two DNA bulks 6 ovary ovl3.1 ovl3.2 both had major effects on with △ (SNP-index) 0.80 (P < 0.01) 0.74 0.01), respectively. Quantitative RT-PCR size-related homologous genes localized consensus FL3.2 conducted. Four candidate exhibited increased expression levels long genotypes. Our results demonstrated power method rapid detection provided reliable regions fine length-related identifying genes.