Quantification of Somatic Chromosomal Rearrangements in Circulating Cell-Free DNA from Ovarian Cancers

• DOI: 10.1038/srep29831
• Publication Date: 2016-07-20T09:24:18Z
• Authors: Faye R. Harris; Irina V. Kovtun; James Smadbeck; Francesco Multinu; Aminah Jatoi; Farhad Kosari; Kimberly R. Kalli; Stephen J. Murphy; Geoffrey C. Halling; Sarah H. Johnson; Minetta C. Liu; Andrea Mariani; George Vasmatzis
• Keywords: Chromosome Aberrations; Ovarian Neoplasms; Reproducibility of Results; DNA, Neoplasm; Real-Time Polymerase Chain Reaction; Sensitivity and Specificity; Article; Tumor Burden; 3. Good health; 03 medical and health sciences; 0302 clinical medicine; Outcome Assessment, Health Care; Humans; Female; Neoplasm Recurrence, Local; Cell-Free Nucleic Acids

Abstract Recently, the use of a liquid biopsy has shown promise in monitoring tumor burden. While point mutations have been extensively studied, chromosomal rearrangements demonstrated greater specificity. Such can be identified and subsequently detected plasma patients using quantitative PCR (qPCR). In this study we used whole-genome mate-pair protocol to characterize landscape genomic primary tumors ten ovarian cancer patients. Individualized tumor-specific primer panels aberrant junctions were for each case by qPCR within cell-free DNA. Selected pre-surgically drawn blood eight Of these eight, three continued presence circulating DNA (ctDNA) post-surgery, consistent with their documented disease, five ctDNA was undetectable post-surgical collection, lack detectable disease. The fraction calculated novel algorithm designed unique challenges quantifying allow observations real-time dynamics. summary, panel individualized derived from could an effective way monitor relapse therapeutic efficacy cfDNA.