Downregulation of the long noncoding RNA GAS5-AS1 contributes to tumor metastasis in non-small cell lung cancer

• DOI: 10.1038/srep31093
• Publication Date: 2016-08-04T09:58:55Z
• Authors: Ying Wu; Hui Lyu; Hongbing Liu; Xuefei Shi; Yong Song; Bolin Liu
• Keywords: GAS5

Abstract Long noncoding RNA (lncRNA) plays pivotal roles in cancer development. To date, only a small number of lncRNAs have been characterized at functional level. Here, we discovered novel lncRNA termed GAS5-AS1 as tumor suppressor non-small cell lung (NSCLC). The expression NSCLC tumors was much lower than that the adjacent normal tissues. reduced significantly correlated with larger tumors, higher TNM stages and lymph node metastasis patients. While ectopic or specific knockdown had no effect on proliferation, cycle progression apoptosis, it dramatically decreased increased, respectively, migration invasion. Overexpression cells cohort molecules (ZEB1, N-cadherin, Vimentin, and/or Snail1) critical for epithelial-mesenchymal transition (EMT). Furthermore, DNA demethylating agent 5-aza-2-deoxycytidine failed to upregulate cells, whereas pan-HDAC inhibitors panobinostat SAHA induced dose-dependent manner. In addition, can be upregulated by HDAC1 HDAC3. Collectively, our data suggest histone modifications play major role leading epigenetic silencing subsequently promote via upregulation several key EMT markers.