Identification of suitable reference gene and biomarkers of serum miRNAs for osteoporosis

• DOI: 10.1038/srep36347
• Publication Date: 2016-11-08T09:33:09Z
• Authors: Jian Chen; Kai Li; Qianqian Pang; Chao Yang; Hongyu Zhang; Feng Wu; Hongqing Cao; Hongju Liu; Yumin Wan; Weibo Xia; Jinfu Wang; Zhongquan Dai; Yinghui Li
• Keywords: Osteopenia; Postmenopausal osteoporosis

Abstract Our objective was to identify suitable reference genes in serum miRNA for normalization and screen potential new biomarkers osteoporosis diagnosis by a systematic study. Two types of models were used like as mechanical unloading estrogen deficiency. Through large-scale screening using microarray, qPCR validation statistical algorithms, we first identified miR-25-3p gene both type osteoporosis, which also showed stability during the differentiation processes osteoblast osteoclast. Then 15 miRNAs with differential expression OVX rats microarray validation. We further detected these postmenopausal women bedrest rhesus monkeys evaluated their diagnostic value ROC analysis. Among miRNAs, miR-30b-5p significantly down-regulated osteopenia or osteoporosis; miR-103-3p, miR-142-3p, miR-328-3p only decreased osteoporosis. They all positive correlations BMD. Except miR328-3p, other three declined after long-duration bedrest. Their AUC values (all >0.75) proved potential. results provided reliable verified group circulating non-invasive detection postmenopausal- unloading-