Single-Multiplex Detection of Organ Injury Biomarkers using SPRi based Nano-Immunosensor

• DOI: 10.1038/srep36348
• Publication Date: 2016-10-31T11:22:10Z
• Authors: Effat Zeidan; Siqi Li; Zhiguo Zhou; Jennifer Miller; Marinella G. Sandros
• Keywords: Multiplex

Abstract The clinical assessment of multiple organ dysfunctions at early stages is recognized to be an important factor in prompting definitive treatment decisions that prevent irreversible damage. In this article, we propose a real-time, label-free, and multiplex nanoenhanced SPRi platform quantitatively assess two biomarkers, kidney injury molecule (KIM-1) high mobility group box-1 (HMGB-1) simultaneously buffer. Our work involves three major contributions the design immunosensor: (1) applied site-specific immobilization antibodies solid surface avoids loss biological activity caused by covalent attachment; (2) constructed well-blocked sensor exhibits minimal non-specific adsorption for singleplex measurements each biomarker buffer; (3) adopted sandwich assay implements functionalized quantum dots (NanoEnhancers) as signal amplifiers achieve sensitivity level 5 pg/mL KIM-1 HMGB-1 We foresee great potential success extending ultra-sensitive variety other emerging biomarkers low concentrations complex matrices.