Mitochondrial Ca2+ uptake controls actin cytoskeleton dynamics during cell migration

• DOI: 10.1038/srep36570
• Publication Date: 2016-11-09T10:08:55Z
• Authors: Julien Prudent; Nikolay Popgeorgiev; Rudy Gadet; Mathieu Deygas; Ruth Rimokh; Germain Gillet
• Keywords: Focal Adhesions; 0303 health sciences; Cell Polarity; Down-Regulation; Article; Mitochondria; Actin Cytoskeleton; 03 medical and health sciences; Cell Movement; Models, Animal; Animals; Calcium; Zebrafish

Abstract Intracellular Ca 2+ signaling regulates cell migration by acting on cytoskeleton architecture, directionality and focal adhesions dynamics. In migrating cells, cytosolic pool pulses are described as key components of these effects. Whereas the role mitochondrial calcium homeostasis Mitochondria Cacium Uniporter (MCU) in were recently highlighted vivo using zebrafish model, their implication actin cystokeleton dynamics mammals is not totally characterized. Here, we show that mcu silencing two human lines compromises capacities. This phenotype characterized stiffness, a polarization loss an impairment adhesion proteins At molecular level, effects appear to be mediated reduction ER pools, which leads decrease Rho-GTPases, RhoA Rac1, -dependent Calpain activites, but seem independent intracellular ATP levels. Together, this study highlights fundamental evolutionary conserved migration.