High-throughput immuno-profiling of mamba (Dendroaspis) venom toxin epitopes using high-density peptide microarrays

• DOI: 10.1038/srep36629
• Publication Date: 2016-11-08T13:06:11Z
• Authors: Mikael Engmark; Mikael R. Andersen; Andreas H. Laustsen; Jigar Patel; Eric Sullivan; Federico de Masi; Christian S. Hansen; Jens V. Kringelum; Bruno Lomonte; José María Gutiérrez; Ole Lund
• Keywords: Antivenom

Abstract Snakebite envenoming is a serious condition requiring medical attention and administration of antivenom. Current antivenoms are antibody preparations obtained from the plasma animals immunised with whole venom(s) contain antibodies against snake venom toxins, but also other antigens. In order to better understand molecular interactions between antivenom epitopes on high-throughput immuno-profiling study all manually curated toxins Dendroaspis species selected African Naja was performed based custom-made high-density peptide microarrays displaying linear toxin fragments. By detection binding for three different performing an alanine scan, elements positions important were identified. A strong tendency recognizing at functional sites observed. With these results, microarray technology first time introduced in field toxinology details evolution antibody-toxin recognition distinctive toxic motifs elucidated.