Evidence for a dynamic role for mononuclear phagocytes during endometrial repair and remodelling

• DOI: 10.1038/srep36748
• Publication Date: 2016-11-09T10:05:05Z
• Authors: Fiona L. Cousins; Phoebe M. Kirkwood; Philippa T. K. Sa...; Douglas A. Gibson
• Keywords: Immunostaining; Monocyte

Abstract In women, endometrial breakdown, which is experienced as menstruation, characterised by high concentrations of inflammatory mediators and immune cells account for ~40% the stromal compartment during tissue shedding. These are known to play a pivotal role in breakdown but their contribution rapid scarless repair endometrium remains poorly understood. current study we used mouse model menstruation investigate dynamic changes mononuclear phagocytes remodelling. Menstruation was simulated MacGreen mice allow visualisation CSF1R + phagocytes. Immunohistochemistry revealed spatio-temporal numbers location CSF1R-EGFP Ly6G neutrophils. Flow cytometry confirmed striking increase GFP (24 h): influxed were 66% F4/80 Gr-1 30% − . Immunostaining identified distinct populations putative ‘classical’ monocytes (GFP ), monocyte-derived macrophages ) stable population tissue-resident - localised areas remodelling respectively. Collectively, these data provide first compelling evidence support different monocytes/macrophages platform future studies on healing.