Superficial vimentin mediates DENV-2 infection of vascular endothelial cells

• DOI: 10.1038/srep38372
• Publication Date: 2016-12-02T10:40:31Z
• Authors: Jie Yang; Lingyun Zou; Yi Yang; Jizhen Yuan; Zhen Hu; Hui Liu; Huagang Peng; Weilong Shang; Xiaopeng Zhang; Junmin Zhu; Xiancai Rao
• Keywords: Protein Conformation, alpha-Helical; 0303 health sciences; Binding Sites; Genetic Vectors; Endothelial Cells; Gene Expression; Dengue Virus; Article; Recombinant Proteins; Cell Line; 3. Good health; Molecular Docking Simulation; 03 medical and health sciences; Aedes; Chlorocebus aethiops; Escherichia coli; Animals; Humans; Receptors, Virus; Protein Conformation, beta-Strand; Protein Interaction Domains and Motifs; Cloning, Molecular; Vero Cells; Protein Binding

Abstract Damage to vascular endothelial cells (VECs) is a critical hallmark of hemorrhagic diseases caused by dengue virus (DENV). However, the precise molecular event involved in DENV binding and infection VECs has yet be clarified. In this study, vimentin (55 kDa) was identified DENV-2 adsorption into VECs. This protein located on surface interacts with envelope domain III (EDIII). The expression level superficial not affected viral or siRNA interference, indicating that exists particular mode. Furthermore, rod mainly functions Molecular docking results predicted several residues EDIII; these may responsible for cell–virus interactions. We propose could novel molecule EDIII directly VEC thus mediates infection.