An air-liquid interphase approach for modeling the early embryo-maternal contact zone

• DOI: 10.1038/srep42298
• Publication Date: 2017-02-09T10:25:32Z
• Authors: S. Chen; S. E. Palma-Vera; M. Langhammer; S. P. Galuska; B. C. Braun; E. Krause; A. Lucas-Hahn; J. Schoen
• Keywords: Oviduct; Interphase

Abstract We developed an air-liquid interphase culture procedure for mammalian oviduct epithelial cells leading to the formation of functional tissues, which generate fluid surrogates. These in vitro epithelia can be co-cultured with living zygotes and enable embryonic development up blastocyst stage without addition embryo medium. The described strategy is broadly applicable analyze early embryo-maternal interactions under standardized conditions.