Hypoxia induces H19 expression through direct and indirect Hif-1α activity, promoting oncogenic effects in glioblastoma

• DOI: 10.1038/srep45029
• Publication Date: 2017-03-22T12:04:45Z
• Authors: Weining Wu; Qi Hu; Er Nie; Tianfu Yu; Youzhi Wu; Tongle Zhi; Kuan Jiang; Feng Shen; Yingyi Wang; Junxia Zhang; Yongping You
• Keywords: Hypoxia; Sp1 transcription factor; U87; Transcription

Abstract H19 expression is elevated in many human tumors including glioblastomas, suggesting an oncogenic role for the long noncoding RNA; yet upregulation of glioblastomas remains unclear. Here we report that hypoxia significantly stimulated glioblastoma cell lines, which was related to hypoxia-inducible factors 1α (Hif-1α). Hif-1α promoted U87 and U251 cells. Meanwhile PTEN advantageous factor affect expression, through attenuating stability. also positively correlates with samples depending on status. ChIP luciferase reporter assays showed induced transcription directly binding promoter. Furthermore, upregulated specific protein 1 (SP1) cells vitro vivo , SP1 strongly interacted promoter promote under hypoxia. We acts as a molecular sponge binds miR-181d, relieving inhibition β-catenin expression. Therefore, participates hypoxia-driven migration invasion In summary, our results uncover mechanisms stimulate malignant effects suggest might be promising therapeutic target.