Protection of Human Colon Cells from Shiga Toxin by Plant-based Recombinant Secretory IgA

• DOI: 10.1038/srep45843
• Publication Date: 2017-04-03T10:49:01Z
• Authors: Katsuhiro Nakanishi; Shota Morikane; Shiori Ichikawa; Kohta Kurohane; Yasuo Niwa; Yoshihiro Akimoto; Sachie Matsubara; Hayato Kawakami; Hirokazu Kobayashi; Yasuyuki Imai
• Keywords: Virulence factor

Abstract Shiga toxin is a major virulence factor of food-poisoning caused by Escherichia coli such as O157:H7. Secretory immunoglobulin (Ig) A (SIgA) supposed to prevent infection the mucosal surface and candidate agent for oral immunotherapy. We previously established recombinant monoclonal antibody (mAb) consisting variable regions from mouse IgG mAb specific binding subunit 1 (Stx1) Fc region IgA. Here we produced secretory form IgA (S-hyIgA) with transgenic Arabidopsis thaliana plant. All S-hyIgA cDNAs ( heavy, light, J chain component ) were expressed under control bidirectional promoter chlorophyll a/b -binding protein A. without using viral promoter. The plant-based exhibited antigen binding, was modified plant-specific N -linked sugar chains. Ig heavy components observed in an intracellular body-like structure leaves on immuno-electron microscopy. An extract neutralized cytotoxicity Stx1 toward butyrate-treated Caco-2 cells, human colon carcinoma cell line. These results will contribute development edible therapeutic antibodies those treatment infection.