Intestinal microbiome in children with severe and complicated acute viral gastroenteritis

• DOI: 10.1038/srep46130
• Publication Date: 2017-04-11T09:23:01Z
• Authors: Shih-Yen Chen; Chi-Neu Tsai; Yun-Shien Lee; Chun-Yuan Lin; Kuan-Yeh Huang; Hsun-Ching Chao; Ming-Wei Lai; Cheng-Hsun Chiu
• Keywords: Sequence Analysis, RNA; Biodiversity; Severity of Illness Index; Article; Rotavirus Infections; Gastroenteritis; Gastrointestinal Microbiome; 3. Good health; Case-Control Studies; RNA, Ribosomal, 16S; Acute Disease; Humans; Child; Caliciviridae Infections

Abstract The aim of the present study was to evaluate microbiota children with severe or complicated acute viral gastroenteritis (AGE). To that end, next-generation sequencing (NGS) technology used sequence 16S ribosomal RNA ( rRNA ) gene in 20 hospitalized pediatric patients AGE and a further otherwise healthy children; fecal microbiome then assessed. Comparative metagenomics data were analyzed by Wilcoxon rank–sum test hierarchical clustering analysis bacterial reads. statistical analyses showed significantly decreased Shannon diversity index (entropy score) intestinal compared normal controls P = 0.017) mild-to-moderate 0.011). score 5 rotavirus lower than those norovirus infection 0.048). Greater richness Campylobacteraceae (P 0.0003), Neisseriaceae 0.0115), Methylobacteriaceae 0.0004), Sphingomonadaceae 0.0221), Enterobacteriaceae 0.0451) found controls. suggest significant reduction microbial AGE, particularly infection.