Fourier transform infrared (FTIR) spectromicroscopic characterization of stem-like cell populations in human esophageal normal and adenocarcinoma cell lines

• DOI: 10.1039/b914311d
• Publication Date: 2009-11-10T12:30:14Z
• Authors: R. Zhao; L. Quaroni; A. G. Casson
• Keywords: Absorbance; Esophageal adenocarcinoma

We have tested an approach to identify putative cancer stem cells that involves measurement of the infrared absorption spectrum individual in aqueous environment, and their subsequent classification using multivariate data analysis techniques. Two primary esophageal cell lines were characterized: immortalized normal epithelial line, Het-1A, adenocarcinoma OE33. In addition, we also evaluated spheroids, reflecting stem-like populations, which derived from each parent line when grown serum-free media. As differences size appeared be a strong discriminating factor, correction needs performed allow reliable based on spectra. demonstrated Het-1A could easily discriminated basis absorbance 1000–1200 cm−1 spectral interval, whereas this was not possible for Furthermore, found changes due aging OE33 culture dominated spectra somewhat limited potential populations vitro model system.