Dual-fluorophore ratiometric pH nanosensor with tuneable pKa and extended dynamic range

• DOI: 10.1039/c1an15042a
• Publication Date: 2011-03-18T09:33:07Z
• Authors: Veeren M. Chauhan; Gary R. Burnett; Jonathan W. Aylott
• Keywords: Nanosensor

Ratiometric pH nanosensors with tuneable pKa were prepared by entrapping combinations of two pH-sensitive fluorophores (fluorescein isothiocyanate dextran (FITC-D) and Oregon Green®dextran (OG-D)) a reference fluorophore (5-(and-6)-carboxytetramethylrhodamine (TAMRA-D)), in biocompatible polymer matrix. Dual-fluorophore permit the measurement an extended dynamic range, from 4.0 to 7.5.