Controlled viable release of selectively captured label-free cells in microchannels

• DOI: 10.1039/c1lc20487d
• Publication Date: 2011-10-14T15:13:00Z
• Authors: Umut Atakan Gurkan; Tarini Anand; Huseyin Tas; David Elkan; Altug Akay; Hasan Onur Keles; Utkan Demirci
• Keywords: Microchannel; Circulating tumor cell

Selective capture of cells from bodily fluids in microchannels has broadly transformed medicine enabling circulating tumor cell isolation, rapid CD4+ counting for HIV monitoring, and diagnosis infectious diseases. Although methods have been demonstrated microfluidic systems, the release captured remains a significant challenge. Viable retrieval label-free will enable new era biological sciences by allowing cultivation post-processing. The challenge comes fact that adhere strongly to microchannel surface, especially when immuno-based immobilization are used. Even though fluid shear enzymes used detach microchannels, these known harm affect cellular characteristics. This paper describes technology selectively without use or enzymes. We successfully released (3.6% mononuclear blood cells) channels with high specificity (89% ± 8%), viability (94% 4%), efficiency (59% 4%). further validated our system specifically capturing controllably releasing CD34+ stem whole blood, which were quantified be 19 per million samples this study. Our results also indicated both healthy amenable vitro culture. Manual flow based method utilizes inexpensive, easy fabricate selective less than 10 minutes, can at point-of-care. presented isolate purify broad spectrum mixed populations offering widespread applications applied sciences, such as tissue engineering, regenerative medicine, rare proteomic/genomic research, clonal/population analyses.