A biosensor fabricated by incorporation of a redox mediator into a carbon nanotube/nafion composite for tyrosinase immobilization: detection of matairesinol, an endocrine disruptor

• DOI: 10.1039/c2an35959f
• Publication Date: 2012-10-26T13:01:06Z
• Authors: Jahangir Ahmad Ra...; Sanaz Pilehvar; Karolien De Wael
• Keywords: Thionine; Nafion

An electrochemical matairesinol biosensor was fabricated by immobilizing tyrosinase on a poly(thionine)/nafion/multi-walled carbon nanotube composite film. A polymeric film of the redox dye thionine enables stable immobilization while acting as mediator for enzymatic process has been incorporated into nanotube/nafion The method is based crosslinking layer with an electropolymerized poly(thionine). good homogenization electron conductor CNTs in integrated films provides possibility three-dimensional conductive network. characterized impedance spectroscopy and characterization. electrode exhibits catalytic activity, high sensitivity, stability applicable over wide range concentrations from 180 nM to 4.33 μM detection limit (LOD) 37 nM. obtained results suggest that developed sensor can be successfully used determination phenolic endocrine disruptors concentration covering their environmental levels.