Backbone modification promotes peroxidase activity of G-quadruplex-based DNAzyme

• DOI: 10.1039/c2cc32919k
• Publication Date: 2012-06-29T08:32:17Z
• Authors: Cong Li; Ling Zhu; Zhi Zhu; Hao Fu; Gareth Jenkins; Chunming Wang; Yuan Zou; Xin Lu; Chaoyong James Yang
• Keywords: Models, Molecular; Binding Sites; Molecular Structure; CATALYSIS; DNA; DNA, Catalytic; 01 natural sciences; 0104 chemical sciences; Enzyme Activation; G-Quadruplexes; Peroxidases; DENSITY; Enzyme Stability; TOPOLOGY; MACHINES

In this work we studied how backbone chemical modifications, such as 2'-O-methyl, phosphorothioate, L-form nucleotides and locked nucleic acid, on G-quadruplex based DNAzymes would affect their peroxidase activity. Our results indicate that 2'-O-methyl modification facilitates the formation of a perfectly compacted parallel structure and significantly promotes peroxidase activity of G-quadruplex based DNAzymes.