Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria

• DOI: 10.1039/c2ee22675h
• Publication Date: 2012-09-27T00:40:15Z
• Authors: Zhengxu Gao; Hui Zhao; Zhimin Li; Xiaoming Tan; Xuefeng Lu
• Keywords: Zymomonas mobilis; Pyruvate decarboxylase

The rapidly growing demand for energy and the environmental concerns about carbon dioxide emissions make development of renewable biofuels more attractive. Tremendous academic industrial efforts have been made to produce bioethanol, which is one major type biofuel. current production bioethanol limited commercialization because issues with food competition (from food-based biomass) or cost effectiveness lignocellulose-based biomass). In this report we applied a consolidated bioprocessing strategy integrate photosynthetic biomass microbial conversion producing ethanol together into bacterium, Synechocystis sp. PCC6803, can directly convert in single biological system. A PCC6803 mutant strain significantly higher ethanol-producing efficiency (5.50 g L−1, 212 mg L−1 day−1) compared previous research was constructed by genetically introducing pyruvate decarboxylase from Zymomonas mobilis overexpressing endogenous alcohol dehydrogenase through homologous recombination at two different sites chromosome, disrupting biosynthetic pathway poly-β-hydroxybutyrate. total, nine dehydrogenases cyanobacterial strains were cloned expressed E. coli test efficiency. effects culturing conditions including tap water, metal ions, anoxic aeration on evaluated.