In situ enzymatic generation of H2O2 from O2 for use in oxidative bleaching and catalysis by TAML activators

• DOI: 10.1039/c3nj00525a
• Publication Date: 2013-07-04T15:02:35Z
• Authors: Jesse A. Miller; Lisa Alexander; Dylan I. Mori; Alexander D. Ryabov; Terrence J. Collins
• Keywords: Tandem; Catalytic cycle; Glucose oxidase; Oxidative enzyme

Iron-TAML activators of peroxides are functional mimics peroxidase and short-circuited cytochrome P450 enzymes that perform numerous transformations appear by all measures to date be fully life cycle compatible with the environment. Here we show how design same catalytic chemistry without direct addition H2O2, thereby removing need transport store hydrogen peroxide. In this new approach, dioxygen is reduced in situ D-glucose presence glucose oxidase (GO) from Aspergillus niger. The resulting tandem TAML–GO system exhibits similar efficiency TAML/H2O2 prototype. shown here efficiently decolorize Orange II oxidize NADH, both near neutral pH (7.5). Computational simulations kinetic data suggest denaturing GO oxidized TAML intermediates does not occur throughout each process. This brings advantage generation low concentrations H2O2 during cycles. minimizes unproductive consumption catalase-like activity iron TAMLs suicidal inactivation. Kinetic for oxidation NADH also reported.