Large-scale generation of cell-derived nanovesicles

• DOI: 10.1039/c4nr02391a
• Publication Date: 2014-08-05T08:31:27Z
• Authors: W. Jo; J. Kim; J. Yoon; D. Jeong; S. Cho; H. Jeong; Y. J. Yoon; S. C. Kim; Y. S. Gho; J. Park
• Keywords: Exosome

Exosomes are enclosed compartments that released from cells and can transport biological contents for the purpose of intercellular communications. Research into exosomes is hindered by their rarity. In this article, we introduce a device uses centrifugal force filter with micro-sized pores to generate large quantity cell-derived nanovesicles. The has simple polycarbonate structure hold filter, operates in common centrifuge. Nanovesicles similar size membrane exosomes. contain intracellular RNAs ranging microRNA mRNA, proteins, plasma proteins. nanovesicles produced using 250 times naturally secreted Also, twice generated murine embryonic stem transfer target cells. Therefore, novel it generates expected be used exosome-related research, applied various applications such as drug delivery cell-based therapy.