A combined toxicity study of zinc oxide nanoparticles and vitamin C in food additives

• DOI: 10.1039/c4nr05480f
• Publication Date: 2014-10-30T09:37:49Z
• Authors: Yanli Wang; Lulu Yuan; Chenjie Yao; Lin Ding; Chenchen Li; Jie Fang; Keke Sui; Yuanfang Liu; Minghong Wu
• Keywords: Nanotoxicology; Nanomaterials; Viability assay

At present, safety evaluation standards for nanofood additives are made based on the toxic effects of a single additive. Since size, surface properties and chemical nature influence toxicity nanomaterials, may have dramatically changed when nanomaterials used as food in complex system. Herein, we investigated combined zinc oxide nanoparticles (ZnO NPs) vitamin C (Vc, ascorbic acid). The results showed that Vc increased cytotoxicity significantly compared with ZnO only NPs. When cells were exposed to NPs at concentration less than 15 mg L(-1), or 300 there was no significant cytotoxicity, both case gastric epithelial cell line (GES-1) neural stem (NSCs). However, L(-1) introduced together, viability decreased sharply indicating cytotoxicity. Moreover, increase also shown vivo experiments. dose study calculated according state nutrition enhancer standard. After repeated oral exposure plus Vc, injury liver kidneys mice has been indicated by change these indices. These findings demonstrate synergistic presented system is essential toxicological assessment nanofood.