An in situ poly(carboxybetaine) hydrogel for tissue engineering applications

• DOI: 10.1039/c6bm00687f
• Publication Date: 2017-01-04T08:53:30Z
• Authors: Hsiu-Wen Chien; Jiashing Yu; Shing Tak Li; Hsin-Yu Chen; Wei-Bor Tsai
• Keywords: 0301 basic medicine; Molecular Structure; Tissue Engineering; Injections, Subcutaneous; Optical Imaging; Biocompatible Materials; Hydrogels; Betaine; Mice; 03 medical and health sciences; Durapatite; Polymethacrylic Acids; Animals; Nanoparticles; Oligopeptides; Cells, Cultured

Hydrogels provide three-dimensional (3D) frames with tissue-like elasticity and high water content for tissue scaffolds. Previously, we reported the design synthesis protocol of a biodegradable poly(carboxybetaine) poly(CB) hydrogel zwitterionic carboxybetaine methacrylate (CBMA) monomer disulfide-containing crosslinker via free radical polymerization. We also demonstrated that cells could be successfully encapsulated in hydrogels without compromising cytoviability. In this study, evaluated cytoviability three commonly used monomers (CBMA, 2-methacryloyloxyethyl phosphorylcholine (MPC) sulfobetaine (SBMA)) suitability being utilized as precursor materials situ gel forming implants. These exhibited lower cell toxicity than other methacrylated monomers. Mixing these dimethacrylate crosslinkers initiated gelation process situ, which was further tested vivo by injecting solutions subcutaneously into murine models. Poly(CB) implants retained their original shape up to 3 weeks, while poly(MPC) poly(SB) shorter periods time due mechanical strengths. showed minimal inflammation at injection site. subsequently CBMA solution mixed Arg-Gly-Asp (RGD) hydroxyapatite (HAp) nanoparticles applied bone engineering. Both vitro studies HAp containing greatly enhanced mineralization formation. The non-cytotoxic conjugated affinity moieties is an excellent material 3D