Readily functionalizable phosphonium-tagged fluorescent coumarins for enhanced detection of conjugates by mass spectrometry

• DOI: 10.1039/c6ob01080f
• Publication Date: 2016-07-21T16:32:15Z
• Authors: Antoine Lizzul-Jurse; Laetitia Bailly; Marie Hubert-Roux; Carlos Afonso; Pierre-Yves Renard; Cyrille Sabot
• Keywords: Phosphonium; Conjugate

Fluorescent coumarins are an important class of small-molecule organic fluorophores ubiquitous in different well-established and emerging fields research including, among others, biochemistry chemical biology. The present work aims at covering the poor detectability coumarin-based conjugates by mass spectrometry while keeping photophysical properties coumarin core. In this context, synthesis readily functionalizable phosphonium-tagged derivatives enabling a dual mass-tag fluorescence labelling analytes or (bio)molecules interest through single-step protocol, is reported. utility these illustrated preparation fluorogenic substrates that facilitated identification peptide fragment released specific proteolytic cleavages.