Surface modification with E-cadherin fusion protein for mesenchymal stem cell culture

• DOI: 10.1039/c6tb00765a
• Publication Date: 2016-05-21T11:40:09Z
• Authors: Yan Zhang; Hongli Mao; Mengyuan Qian; Feifei Hu; Lei Cao; Ke Xu; Qizhi Shuai; Chao Gao; Ren Lang; Toshihiro Akaike; Jun Yang
• Keywords: 0301 basic medicine; 03 medical and health sciences; 3. Good health

To effectively expand human mesenchymal stem cells (hMSCs) in vitro without affecting their innate biological properties, a fusion protein (hE-cad-Fc) consisting of E-cadherin extracellular domain and an immunoglobulin G Fc region was fabricated used as biomimetic matrix for MSC culture surface modification. The results showed that cultured on hE-cad-Fc-modified polystyrene surfaces exhibited improved proliferation paracrine functions compared with unmodified collagen-modified surfaces. Meanwhile, modified hE-cad-Fc inhibited cell apoptosis even under the serum deprivation conditions. Additionally, not only up-regulated expression β-catenin MSCs stimulated cellular membrane complex E-cadherin/β-catenin, but also activated intracellular signals such EGFR, AKT ERK phosphorylation. Therefore, appeared to be promising candidate modification culture.